Applied Biological Materials (abm)

Immortalized Mouse Kidney Epithelial Cells

Product Code:
ABM-T0085
Product Group:
Cell Lines
Host Type:
Mouse
Regulatory Status:
RUO
 

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Further Information

Description:
The Immortalized Mouse Kidney Epithelial Cells (HPV E6/E7) are derived by infection of primary mouse kidney cells with lentivirus HPV E6/E7. This cell line maintains its primary phenotype and can be grown extensively. While the Immortalized Mouse Kidney Epithelial Cells have been used to study diabetes, its primary counterpart have been used to study inflammatory diseases, intrinsic kidney repair mechanisms and acute renal failures. Real Time PCR was used to quantify the HPV gene expression in immortalized cell line.
Donor History:
10 day old post natal mice
Growth Conditions:
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. TM0085 + 2.5% FBS + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO2.
Growth Properties:
Adherent, epithelial
Immortalisation Method:
Transduction with lentiviral HPV (abm Cat. No. LV617).
Markers:
E-cadherin, CK8 and CK18
Population Doubling:
35-45
Seeding Density (/cm^2):
5,000 - 10,000
Split Ratio:
1:2 to 1:3
Tissue:
Kidney