Applied Biological Materials (abm)

Immortalized Human Pulmonary Microvascular Endothelial Cells

Product Code:
ABM-T0491
Product Group:
Cell Lines
Host Type:
Human
Regulatory Status:
RUO
 

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Further Information

Description:
The pulmonary microvascular endothelial cells (PMEC) are isolated from human pulmonary arteries. The PMECs form a semiselective barrier that is critical for lung gas exchange and regulation of fluid and solute passage between the blood and interstitial compartments in the lung. HPAEC have been used for the study of vascular permeability and inflammatory responses. HPAEC in co-culture with HPASMC have been used as a model for pulmonary angiopathy. Real Time PCR was used to quantify transgene expression.
Donor History:
Not disclosed
Growth Conditions:
Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is recommended for cell adhesion to the culture vessels. PriGrow I (TM001) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO2.
Growth Properties:
Adherent, polygonal
Immortalisation Method:
Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen
Seeding Density (/cm^2):
50,000
Tissue:
Blood Vessel

References

Huo, Z., Kong, Y., Meng, M., Cao, Z., Zhou, Q. (2019). Atorvastatin enhances endothelial adherens junctions through promoting VE_PTP gene transcription and reducing VE-cadherin-Y731 phosphorylation. Vascular Pharmacology, 117, 7-14. doi:10.1016/j.vph.2018.06.003