Applied Biological Materials (abm)

Immortalized Human Prostate Epithelial Cells

Product Code:
 
ABM-T0600
Product Group:
 
Cell Lines
Host Type:
 
Human
Regulatory Status:
 
RUO
 

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CodeSizePrice
ABM-T06001x10^6 cells / 1.0 mlEnquire
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Further Information

Description:
1. Real Time PCR was used to quantify the transgene expression
Donor History:
Male, 20, Caucasian
Growth Conditions:
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Prigrow X Series Medium (TM0600), 37.0°C, 5% CO2. Note: PriGrow X Series Medium for T0600 (Cat. No. TM0600) is serum-free. Thaw cells in media supplemented with 10% FBS to promote attachment to the vessel, then change thawing media to serum-free TM0600 media within 24 hours, once attachment is observed.Cells are sensitive to trypsin; 1/10 diluted 0.25% Trypsin (TM050) or Gentle Dissociation Solution (TM080) is recommended for subculture procedures. 
Growth Properties:
Adherent, polygonal
Immortalisation Method:
Serial passaging and transduction with recombinant lentiviruses carrying SV40 whole gene and HPV E6/E7 gene
Markers:
Puromycin-resistance
Population Doubling:
25 - 35
Seeding Density (/cm^2):
400,000 - 800,000
Split Ratio:
1:2 to 1:5
Tissue:
Prostate