Applied Biological Materials (abm)

Immortalized Human Neonatal Foreskin Keratinocytes (HKc16E6/E7-II)

Product Code:
 
ABM-T0726
Product Group:
 
Cell Lines
Host Type:
 
Human
Regulatory Status:
 
RUO
 

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ABM-T07261x10^6 cells / 1.0 mlEnquire
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Further Information

Description:
Immortalized Human Neonatal Foreskin Keratinocytes (HKc16E6/E7-II) are normal human keratinocytes isolated from neonatal foreskins biopsy and immortalized by transfection with HPV16 E6 and E7 genes. HKc16E6/E7-II cells will be useful for investigating the molecular biology of HPV16 and the interactions between the viral DNA and the human genome. HKc16E6/E7-II cell line can also serve a model for analyzing the effects of HPV16 on the function and pathology of human epithelial cells. Characterization by RNaseH assays, ODN uptake measurements, Western blot, and RT-PCR.
Donor History:
Not disclosed
Growth Conditions:
Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow X Series Medium (TM0726), 37.0°C, 5% CO2
Growth Properties:
Adherent, epithelial-like
Immortalisation Method:
Transfection with HPV16 E6 and E7
Markers:
HPV-16 E6/E7, neomycin and ampicillin resistance
Tissue:
Skin

References

Alvarez-Salas, L. M., Arpawong, T. E., & DiPaolo, J. A. (1999). Growth inhibition of cervical tumor cells by antisense oligodeoxynucleotides directed to the human papillomavirus type 16 E6 gene. Antisense & nucleic acid drug development, 9(5), 441?450. https://doi.org/10.1089/oli.1.1999.9.441