Applied Biological Materials (abm)

Immortalized Human Middle Ear Epithelial Cell Line (ROM1)

Product Code:
ABM-T0413
Product Group:
Cell Lines
Host Type:
Human
Regulatory Status:
RUO
 

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Further Information

Description:
These immortalized middle ear cell lines (Cat. T0412 - T0146) are derived from donors with cochlear implants (T0412), recurrent otitis media (T0413, T0414) and otitis media with effusion (T0415, T0416). PCR were used for marker detection and cells were validated by cytogenetic analysis. These cell lines are suitable research models for investigating the molecular pathogenesis of middle ear diseases including otitis media. Complete list of cell lines in this panel: Cat. T0412 - Immortalized Human Middle Ear Epithelial Cell Line (PCI)Cat. T0413 - Immortalized Human Middle Ear Epithelial Cell Line (ROM1)Cat. T0414 - Immortalized Human Middle Ear Epithelial Cell Line (ROM2)Cat. T0415 - Immortalized Human Middle Ear Epithelial Cell Line (OME1)Cat. T0416 - Immortalized Human Middle Ear Epithelial Cell Line (OME2)
Growth Conditions:
PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. PriGrow X Series Medium (TM0413) + 0.25ug/mL Amphotericin B (G274) + 1% Penicillin/Streptomycin Solution (G255), 33.0°C, 5% CO2. Change media every 2-3 days.
Growth Properties:
Adherent, cobble-stone polygonal
Markers:
TNFA, IL1B, IL6, IL8, MUC5AC, MUC5B
Population Doubling:
48-72
Seeding Density (/cm^2):
13,000
Split Ratio:
01:04
Tissue:
Ear

References

Espahbodi M, Samuels TL, McCormick C, Khampang P, Yan K, Marshall S, McCormick ME, Chun RH, Harvey SA, Friedland DR, Johnston N, Kerschner JE. Analysis of Inflammatory Signaling in Human Middle Ear Cell Culture Models of Pediatric Otitis Media. Laryngoscope. 2021 Feb;131(2):410-416. doi: 10.1002/lary.28687. Epub 2020 May 20. PMID: 32433794.Blaine-Sauer S, Samuels TL, Khampang P, Yan K, McCormick ME, Chun RH, Harvey SA, Friedland DR, Johnston N, Kerschner JE. Establishment of Novel Immortalized Middle Ear Cell Linesas Models for Otitis Media. Laryngoscope, Submitted Jan 19 2023.