Applied Biological Materials (abm)

Immortalized Human Epidermal Keratinocytes (SIK)

Product Code:
ABM-T0770
Product Group:
Cell Lines
Host Type:
Human
Regulatory Status:
RUO
 

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Further Information

Description:
The Immortalized Human Epidermal Keratinocytes (SIK) were derived from a sample of human foreskin and the serial cultivation of keratinocytes led to its spontaneous immortalization. The immortalized cells resemble normal human epidermal colonies but with dramatically increased levels of cell cycle regulatory proteins such as cyclin A. Treatment of the Immortalized Human Epidermal Keratinocytes (SIK) with either TGF-f or TPA were highly effective in preventing growth in low calcium medium which is similar to normal keratinocytes. Telomerase activity in the immortalized cells is stimulated approximately 5-fold by cultivation in the presence of epidermal growth factor (EGF). The Immortalized Human Epidermal Keratinocytes (SIK) is useful in studies focusing on dermatology particularly in skin carcinogenesis as telomerase activation in human skin has been found to be associated with exposure to ultraviolet radiation.
Donor History:
Male
Growth Conditions:
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow X Series Medium (TM0770), 37.0°C, 5% CO2.
Growth Properties:
Adherent, polygonal
Immortalisation Method:
Spontaneous immortalization
Tissue:
Skin

References

Rice, R. H., Steinmann, K. E., deGraffenried, L. A., Qin, Q., Taylor, N., & Schlegel, R. (1993). Elevation of cell cycle control proteins during spontaneous immortalization of human keratinocytes. Molecular biology of the cell, 4(2), 185?194. https://doi.org/10.1091/mbc.4.2.185