Applied Biological Materials (abm)

Immortalized Human Delta F508 Cystic Fibrosis Cells

Product Code:
 
ABM-T0738
Product Group:
 
Cell Lines
Host Type:
 
Human
Regulatory Status:
 
RUO
 

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ABM-T07381x10^6 cells / 1.0 mlEnquire
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Further Information

Description:
This Immortalized Human Delta F508 Cystic Fibrosis Cells are dervied from bronchial epithelial primary cells isolated from a patient with Delta F508 Cystic Fibrosis. The cells were immortalized by external manipulated to express BMI-1 and hTERT. These cells have a diploid karyotype and electrophysiologic properties. This cell line is applicable for research relating to cystic fibrosis and other applications.
Donor History:
Female, 54, Delta F508 Cystic Fibrosis
Growth Conditions:
PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. PriGrow X Series Mediuam (TM0738), 37.0°C, 5% CO2 Note: Cells are sensitive to trypsin; Gentle Dissociation Solution (TM080) is recommended for subculture procedures. 
Growth Properties:
Adherent, epithelial-like
Immortalisation Method:
Bmi-1 and the catalytic subunit of telomerase (hTERT).
Population Doubling:
18 - 24
Seeding Density (/cm^2):
15,000 - 20,000
Split Ratio:
01:04
Tissue:
Airway

References

Fulcher, M. L., Gabriel, S. E., Olsen, J. C., Tatreau, J. R., Gentzsch, M., Livanos, E., Saavedra, M. T., Salmon, P., & Randell, S. H. (2009). Novel human bronchial epithelial cell lines for cystic fibrosis research. American journal of physiology. Lung cellular and molecular physiology, 296(1), L82?L91. https://doi.org/10.1152/ajplung.90314.2008 Gentzsch, M., Boyles, S. E., Cheluvaraju, C., Chaudhry, I. G., Quinney, N. L., Cho, C., Dang, H., Liu, X., Schlegel, R., & Randell, S. H. (2017). Pharmacological Rescue of Conditionally Reprogrammed Cystic Fibrosis Bronchial Epithelial Cells. American journal of respiratory cell and molecular biology, 56(5), 568?574. https://doi.org/10.1165/rcmb.2016-0276MA