Applied Biological Materials (abm)

Immortalized Human Corneal Keratinocytes (IHCK)

Product Code:
 
ABM-T0579
Product Group:
 
Cell Lines
Host Type:
 
Human
Regulatory Status:
 
RUO
 

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ABM-T05791x10^6 cells / 1.0 mlEnquire
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Further Information

Description:
Corneal Keratinocytes play a major role in maintaining the balance of stromal substances and preserving the corneal transparency. Established using the HPV16 E6/E7 genes, The Immortalized Human Corneal Keratinocytes (IHCK) not only retain their authentic cell biomarkers (CK12, CK19), but also displays fairly disomic cytogenetic status at chromosomes 1, 8, 10 and 18, which is an indication of normal diploid chromosomal status. Together with the Immortalized Human Corneal Stromal Fibroblasts (T0578) and the Immortalized Human Corneal Endothelial Cells (T0577) - isolated from the same donor and immortalized using the same platform - this uniform model system presents a valuable tool for studies involving cornea homeostasis and ophthalmologic diseases.
Donor History:
Not disclosed
Growth Conditions:
PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. PriGrow X Series Medium (TM0579), 37.0°C, 5% CO2Note: Cells are sensitive to trypsin; Gentle Dissociation Solution (TM080) is recommended for subculture procedures. 
Growth Properties:
Adherent, spindle-shaped
Immortalisation Method:
Serial passaging and transduction with retroviruses carrying human papillomavirus type 16 E6/E7 gene.
Markers:
CK19, CK12, ABCG2, Neomycin-resistant
Population Doubling:
24
Seeding Density (/cm^2):
30,000 - 50,000
Tissue:
Eye

References

Schulz, S., Steinberg, T., Beck, D., Tomakidi, P., Accardi, R., Tommasino, M., Reinhard, T., & Eberwein, P. (2013). Generation and evaluation of a human corneal model cell system for ophthalmologic issues using the HPV16 E6/E7 oncogenes as uniform immortalization platform. Differentiation; research in biological diversity, 85(4-5), 161?172. https://doi.org/10.1016/j.diff.2013.06.001