Applied Biological Materials (abm)

Immortalized Human Bronchial Epithelial Cells (HBEC3-KT) - Cdk4/hTERT

Product Code:
ABM-T0753
Product Group:
Cell Lines
Host Type:
Human
Regulatory Status:
RUO
 

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Further Information

Description:
Immortalized Human Bronchial Epithelial Cells (HBEC3-KT) - Cdk4/hTERT were isolated and immortalized from bronchial biopsy specimens using Cdk4 and hTERT expression vectors. It serves as a model that can be genetically manipulated in a stepwise fashion to progress the cells toward malignancy that may be useful for studying the early stages of preneoplasia and progression, differentiation, and morphogenesis and for comparisons with lung cancer for the identification of new genetic and epigenetic differences between tumor and normal cells.Characterization by PCR, immunocytochemistry, karyotyping, and microarray.
Donor History:
Not disclosed
Growth Conditions:
Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow X Series Medium (TM0753), 37.0°C, 5% CO2
Growth Properties:
Adherent, epithelial
Immortalisation Method:
Immortalized with pSRαMSU expressing Cdk4 and pBABE expressing hTERT
Markers:
Puromycin and neomycin resistance
Population Doubling:
32 - 42
Seeding Density (/cm^2):
30,000 - 40,000
Tissue:
Airway

References

Ramirez, R. D., Sheridan, S., Girard, L., Sato, M., Kim, Y., Pollack, J., ? Minna, J. D. (2004). Immortalization of Human Bronchial Epithelial Cells in the Absence of Viral Oncoproteins. Cancer Research, 64(24), 9027?9034. doi:10.1158/0008-5472.can-04-3703Blackman, M. C., Capeloa, T., Rondeau, J. D., Zampieri, L. X., Benyahia, Z., Van de Velde, J. A., ... & Sonveaux, P. (2022). Mitochondrial Protein Cox7b Is a Metabolic Sensor Driving Brain-Specific Metastasis of Human Breast Cancer Cells. Cancers, 14(18), 4371.