Oxalate (Oxalic Acid) Colorimetric Assay Kit (Extraction method)

2 h 30 min

Microplate reader

0.006-1 mmol/L

Colorimetric method

4.5-7.2%

2.1-3.2%

Quantitative

0.006 mmol/L

Oxalic, also known as ethanedioic acid, is a simple organic acid in plants and plays important physiological roles. Oxalic participates in the regulation of Ca²⁺, pH and osmotic pressure within cells, as well as the defense responses of plant pathogens and the alleviation of aluminum toxicity. Although Oxalic has important physiological functions, excessive intake of Oxalic can also cause many adverse effects on the human body. Oxalic affects the absorption of mineral elements such as calcium and iron by the human body, increases the risk of developing kidney stones, inflammation, hyperoxaluria, uremia and other diseases, and in severe cases, it can even cause acute Oxalic poisoning. Moreover, oxalic is not easily oxidized and decomposed in the human body, and its metabolites can disrupt the acid-base balance in the body.
The detection principle of this kit: Oxalate oxidase catalyzes the oxidation of oxalate to produce hydrogen peroxide and carbon dioxide. Hydrogen peroxide reacts with chromogenic substances under the action of enzymes to form colored products. There is a maximum absorption peak at 550 nm, and the color depth is proportional to the content of oxalic.

Serum;Plasma;Animal and plant tissue;Urine