Elabscience

Hep G2 Cell Line

Product Code:
CL-0103
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
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Further Information

Age Gender:
15 years adolescent
Applications:
These cells are suitable as a transfection host.
Background:
The cells express 3-hydroxy-3-methylglutaryl-CoA reductase and hepatic triglyceride lipase activities.The cells demonstrate decreased expression of apoA-I mRNA and increased expression of catalase mRNA in response to gramoxone(oxidative stress).There is no evidence of a Hepatitis B virus genome in this cell line.
Biosafety Level:
1
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
MEM(PM150410)+10% FBS+1% P/S(PB180120)
Effects:
No, in immunosuppressed mice; Yes, in semisolid medium.
Gene expression:
Alpha-Fetoprotein(Alpha Fetoprotein); Albumin; Alpha2 Macroglobulin(Alpha-2-Macroglobulin); Alpha1 Antitrypsin(Alpha-1-Antitrypsin); Transferrin; Alpha1 Antichymotrypsin;(Alpha-1-Antichymotrypsin); Haptoglobin; Ceruloplasmin; Plasminogen;, Complement(C4);
Growth properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C
Medium Renewal:
Every 2 to 3 days
Morphology:
Epithelial
Receptor expression:
Insulin; Insulin-like growth factor II(IGF II)
Subcultivation Ratio:
1:2 to 1:4
Subculturing:
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Tissue:
Liver
Tumorigenic:
No

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