Elabscience

HL-60 Cell Line

Product Code:
CL-0110
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
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Further Information

Age Gender:
36 years
Applications:
Transfection.
Background:
HL-60 cells spontaneously differentiate and differentiation can be stimulated by butyrate, Hypoxanthine, phorbol myristic acid(PMA, TPA), dimethylsulfoxide(DMSO, 1% to 1.5%), actinomycin D, and retinoic acid.The cells exhibit phagocytic activity and responsiveness to chemotactic stimuli.The line is positive for myc oncogene expression.
Biosafety Level:
1
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
IMDM(PM150510)+20% FBS+1% P/S(PB180120)
Effects:
Yes, in nude mice(subcutaneous myeloid tumors). Yes, in semi-solid media.
Gene expression:
Tumor Necrosis Factor(Tnf), Also Known As Tumor Necrosis Factor Alpha(Tnf-Alpha, Tnf Alpha), After Stimulation With Phorbol Myristic Acid, Myc+
Growth properties:
Suspension
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C
Medium Renewal:
Every 2 to 3 days
Morphology:
Myeloblast
Receptor expression:
Complement, expressed; Fc, expressed
Subcultivation Ratio:
1:2 to 1:4
Subculturing:
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2?10^5 viable cells/ml. Maintain cell density between 1?10^5 and 1?10^6 viable cells/ml.
Tissue:
Peripheral blood
Tumorigenic:
Yes

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