Elabscience

HMy2.CIR cell line

Product Code:
CL-0112
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
33 years
Background:
This cell line is established by gamma irradiation and selection for loss of HLA class I antigen expression from the HMy.2 B lymphoblastoid cell line, a fast growing mutant of the ARH-77 cell line. The cells can be utilized as host for transfected class I major histocompatibility antigen genes. The ARH-77 cell line was reported to be positive for Epstein-Barr nuclear antigen (EBNA +) and Epstein-Barr viral capsid antigen (EBVCA +). Therefore, the HMy2.CIR cell line is assumed to be EBNA positive. The cells express small amounts of HLA Cw4 but no HLA A or B locus products.
Biosafety Level:
2
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
IMDM(PM150510)+10% FBS+1% P/S(PB180120)
Growth properties:
Suspension
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C
Medium Renewal:
Every 2 to 3 days
Morphology:
Lymphoblast
Subcultivation Ratio:
2?10^5 to 1?10^6 cells/mL
Subculturing:
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2?10^5 viable cells/ml. Maintain cell density between 2?10^5 and 1?10^6 viable cells/ml.
Tissue:
B-Lymphoblast

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