MSTO-211H cell line

male, 62 years

This cell line was established in 1985 from the the pleural effusion of metastatic site on biphasic mesothelioma. The 62-year-old male Caucasian patient had not received prior radiation or chemotherapy. The cells have EGF binding sites with high binding affinity. The cells express alpha- and beta-subunits of human chorionic gonadotropin as well as Neuron specific enolase. L-DOPA decarboxylase, bombesin and neurotensin were not detected in the cells. C-myc protooncogene is overexpressed by the cells with no gene rearrangement/amplification observed. The cells are positive for the expression of v-src, v-abl, v-erb B, c-raf 1, Ha-ras, Ki-ras, and N-ras. No detectable expression of N-myc, L-myc, c-myb, c-fos, v-fes, v-fms and v-sis. A saturation cell density of 400,000 cell/cm2 can be reached with cell surface slough off.

1

Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)

Adherent

Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C

Every 2 to 3 days

Fibroblast-Like

1:3 to 1:4

Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Lung; Derived From Metastatic Site: Pleural Effusion

Yes, tumors for med in approximately 20% of nude mice inoculated with MSTO-211H cells.