Elabscience

Neuro-2a [N2a; Neuro-2a] Cell Line

Product Code:
CL-0168
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Mouse
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
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Further Information

Antigen expression:
H-2, a haplotype; Mus musculus, expressed
Applications:
Transfection. The cell line has been used for studies on the mechanism of vinblastine precipitation of microtubular protein, the kinetics of GTP binding to isolated protein, the turnover of microtubules in vivo, and the synthesis and assembly of microtubu
Background:
Neuro-2a cells produce large quantities of microtubular protein which is believed to play a role in a contractile system which is responsible for axoplasmic flow in nerve cells. Tested and found negative for ectromelia virus(mousepox).
Biosafety Level:
1
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSOStorage temperature: Liquid nitrogen vapor phase
Culture medium:
MEM(PM150410)+10% FBS+1% P/S(PB180120)
Gene expression:
Acetylcholinesterase, Tubulin
Growth properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5%Temperature: 37°C
Medium Renewal:
Every 2 to 3 days
Morphology:
Neuronal and amoeboid stem cells
Subcultivation Ratio:
1:2 to 1:4
Subculturing:
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Tissue:
Brain

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