Elabscience

NIH:OVCAR-3 [OVCAR3] Cell Line

Product Code:
CL-0178
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
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Further Information

Age Gender:
60 years
Applications:
transfection. OVCAR3 is an appropriate model system in which to study drug resistance in ovarian cancer and the presence of hormone receptors should be useful for the evaluation of hormonal therapy.
Background:
Forms colonies in soft agar and has an abnormal karyotype.Resistant to clinically relevant concentrations of adriamycin, melphalan and cisplatin.Both cultured cells and xenografts exhibit androgen and estrogen receptors.Xenograft models have been used to show that treatment with 17 beta Estradiol can induce progesterone receptors in this human ovarian carcinoma.
Biosafety Level:
1
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSOStorage temperature: Liquid nitrogen vapor phase
Culture medium:
RPMI-1640(PM150110)+20% FBS+0.01mg/ml Insulin+1% P/S(PB180120)
Effects:
Yes, Forms colonies in soft agar; Yes, in nude mice inoculated subcutaneously with 10^7 cells. Tumors developed within 21 days at 100% frequency(5/5).
Growth properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5%Temperature: 37°C
Medium Renewal:
Every 2 to 3 days
Morphology:
Epithelial
Receptor expression:
Androgen receptor, positive; estrogen receptor, positive; progesterone receptor, positive
Subcultivation Ratio:
1:2 to 1:4
Subculturing:
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Tissue:
Ovary
Tumorigenic:
Yes

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