Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
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Further Information
59 years
cytokeratin-18;Homo sapiens, expressed
p53 antigen;Homo sapiens, expressed
prostate specific antigen (PSA) ;Homo sapiens, expressed
prostatic acid phosphatase (PAP) ;Homo sapiens, expressed
Rb protein;Homo sapiens, expressed
Recently, it has been shown that VCaP prostate cancer cells produce the mouse xenotropic retrovirus Bxv-1, which was likely acquired by the cells during their xenotransplantation in mice.
2
Freeze Medium: 55% Basal Medium+40% FBS+15% DMSO
Storage temperature: Liquid nitrogen vapor phase
DMEM (PM150210)+10% FBS+1% P/S (PB180120)
Yes, in nude and SCID mice.
cytokeratin-18;Homo sapiens, expressed, p53 antigen;Homo sapiens, expressed, prostate specific antigen (PSA) ;Homo sapiens, expressed, prostatic acid phosphatase (PAP) ;Homo sapiens, expressed, Rb protein;Homo sapiens, expressed
Adherent
Atmosphere: Air, 95%; CO2, 5%;
Temperature: 37°C
every 2 to 3 days
Epithelial
1:2 to 1:4
Prostate, derived from metastatic site: vertebral metastasis
Yes