Elabscience

Y3-Ag 1.2.3 cell line

Product Code:
CL-0297
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Rat
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Background:
This cell line is derived from an azaguanine-resistant mutant of S210 myeloma. The cells are resistant to 8-azaguanine but sensitive to HAT. The cells can be used as a rat B cell fusion partner for preparing rat-rat hybridomas. Commercial use or third party distribution has to be permitted by C. Milstein.
Biosafety Level:
1
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
DMEM(PM150210)+10% FBS+1% P/S(PB180120)
Gene expression:
Immunoglobulin
Growth properties:
Suspension
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C
Medium Renewal:
Every 2 to 3 days
Morphology:
Lymphoblast
Subcultivation Ratio:
1?10^5 to 1?10^6 cells/mL
Subculturing:
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.
Tissue:
Disease: Plaemocytoma; Myeloma Strain: Lou; Cell Type: B Lymphoblastoma

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