Elabscience

NCI-H209 [H209] cell line

Product Code:
CL-0393
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
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Further Information

Age Gender:
55 years
Background:
This cell line was established in 1979 from the bone marrow aspirated of a 55-year-old male Caucasian with small cell lung carcinoma prior to treatment. The line is a classic small cell lung carcinoma cell line that expresses four biochemical markers, i.e., neuron specific enolase, brain isoenzyme of creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity.
Biosafety Level:
1
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)
Growth properties:
Suspension
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C
Medium Renewal:
2 to 3 times per week
Morphology:
Round Cell Clusters
Subcultivation Ratio:
1:2 to 1:3
Subculturing:
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:3.
Tissue:
Lung; Originating From Metastatic Site: Bone Marrow
Tumorigenic:
Yes, forms transplantable tumors with typical SCLC histology in nude mice.

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