Elabscience

NCI-H23 cell line

Product Code:
CL-0397
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
male, 51 years
Background:
This line was established from lung cancer tissue obtained from a 59-year-old black male lung adenocarcinoma patient prior to therapy. The cells carry the mutation of K-ras 12 and a mutation in the codon 246 (ATC &8594;ATG, isoleucine -> methionine) of the p53 gene. The cells express C-myc, L-myc, v-src, v-abl, v-erb B, c-raf 1, Ha-ras, Ki-ras and N-ras RNAs. The cell line has a heterogeneous mRNA expression for PDGF A and B chain, transforming growth factor alpha and beta and the epidermal growth factor receptor (EGFR). NCI-H23 exhibits a 20-fold higher level of c-myc DNA amplification without detectable c-myc RNA amplification. The cells stain positive for keratins 5+8 and 18 and vimentin but negative for neurofilament. The cells are L-dopa decarboxylase-negative. The cells can form colonies in soft agarose with an efficiency of 9.7%.
Biosafety Level:
1
Cryopreservation:
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture medium:
RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)
Gene expression:
myc+; src+; abl+; erb+; ras+; sis -
Growth properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5% Temperature: 37°C
Medium Renewal:
Every 2 to 3 days
Morphology:
Epithelial
Subcultivation Ratio:
1:3 to 1:4
Subculturing:
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Tissue:
Lung

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