Elabscience

NIH/3T3

Product Code:
CL-0171
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Mouse
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Age Gender:
Embryo;Male
Background:
NIH/3T3 cells are a continuously passaged cell line with strong contact inhibition, established from cultures of NIH Swiss mouse embryos. To develop subclones that are more suitable for transformation studies based on their morphological traits, the established NIH/3T3 cell line underwent more than five rounds of subcloning. NIH/3T3 cells are highly sensitive to the focus formation caused by sarcoma viruses and to the propagation of leukemia viruses, making them very useful for DNA transformation and transfection studies; NIH/3T3 cells are negative for mousepox virus.
Biosafety Level:
BSL-1
Cryopreservation:
Cryopreservation solution: serum-free, non-programmed cryopreservation solution [PB180438]
Culture medium:
DMEM [PM150210]+10% FBS [164220]+1% P/S [PB180120]
Growth properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5%, Temperature: 37°C
Medium Renewal:
every 2 to 3 days
Morphology:
fibroblast-like
Subcultivation Ratio:
1:3-1:6
Tissue:
Embryo

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