Elabscience

AMC-HN-8 Cell Line

Product Code:
CL-1160
Product Group:
Cell Lines
Supplier:
Elabscience
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Dry Ice
Storage:
For long-term cryopreservation, cryovials should be stored in liquid nitrogen at -150°C to -196°C. Storage at -80°C is restricted to short-term interim use only.
 

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This product comes from: China.
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Further Information

Age Gender:
46Y, Male
Background:
AMC-HN-8 is a head and neck cancer cell line established in 1996 at Asan Medical Center, as one of nine cell lines derived from primary or metastatic tumor tissues of patients with head and neck malignancies. Immortalized through primary tissue isolation and in vitro expansion, AMC-HN-8 grows as adherent, epithelial-like monolayers. Originating from squamous cell carcinoma, this cell line presents high expression of β4 integrin and is frequently subjected to flow cytometry and indirect immunofluorescence assays to evaluate cellular DNA content and the expression of integrin subunits α6, β1, β4, as well as intercellular adhesion molecule-1 (ICAM-1). AMC-HN-8 is widely utilized for nude mouse xenograft tumor formation and histological analysis, serving as a classic in vitro model for biological research on head and neck tumors.
Biosafety Level:
BSL-1
Cryopreservation:
General Freezing Medium [PB180436]
Culture medium:
RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Dissociation Duration:
4-5 min
Growth properties:
Adherent
Incubation Atmosphere:
Atmosphere: Air, 95%; CO2, 5%, Temperature: 37°C
Medium Renewal:
2 to 3 times per week
Morphology:
Epithelial-like
Subcultivation Ratio:
1:2-1:4
Subculturing:
1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ?g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Tissue:
Larynx; derived from metastatic site: lymph node

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