PARP-1 [ARTD1] (E988K Mutant) (human) (rec.) Control
Product Code:
AG-40T-0015
AG-40T-0015
Regulatory Status:
RUO
RUO
Target Species:
Human
Human
Shipping:
Dry Ice
Dry Ice
Storage:
Short term: -20°C. Long term: -80°C
Short term: -20°C. Long term: -80°C
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| Code | Size | Price |
|---|
| AG-40T-0015-C010 | 10 ug | £157.00 |
Quantity:
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This product comes from: Switzerland.
Typical lead time: 7-10 working days.
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Typical lead time: 7-10 working days.
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- Further Information
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Further Information
Alternate Names/Synonyms:
Poly(ADP-ribose) Polymerase 1; ADP-ribosyltransferase Diphtheria Toxin-like 1; NAD(+) ADP-ribosyltransferase 1
Biological Activity:
0.5% of wild type PARP-1.
Concentration:
Lot dependent (0.2-0.5mg/ml)
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride and 50mM imidazole, 0.2% NP-40 and 10% glycerol.
Handling Advice:
After opening, prepare aliquots and store at -80°C.Avoid freeze/thaw cycles.
Long Description:
Protein. Human full-length inactive mutant E998K of PARP-1 is fused to a HA-tag and a His-tag. Source: Sf21 cells. Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride and 50mM imidazole, 0.2% NP-40 and 10% glycerol. Purity: >95% (SDS-PAGE). PARP-1 (ARTD1) is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PARP-1 positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. It forms a complex with EEF1A1 and TXK that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. PARP-1 (E998K mutant) is an inactive form of PARP-1 which can be used as a control compound.
NCBI, Uniprot Number:
P09874
Package Type:
Plastic Vial
Product Description:
PARP-1 (ARTD1) is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PARP-1 positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. It forms a complex with EEF1A1 and TXK that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. PARP-1 (E988K mutant) is an inactive form of PARP-1 which can be used as a control compound.
Purity:
>95% (SDS-PAGE)
Sequence:
Human full-length inactive mutant E988K of PARP-1 is fused to a HA-tag and a His-tag.
Source / Host:
Sf21 cells
TAGs:
His
Transportation:
Non-hazardous
UNSPSC Category:
Other Proteins
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 6 months after receipt when stored at -80°C.
Documents
References
The enzymatic and DNA binding activity of PARP-1 are not required for NF-kappa B coactivator function: P.O. Hassa, et al.; J. Biol. Chem. 276, 45588 (2001)