AdipoGen Life Sciences

PARG (human) (rec.) (His) (highly active)

Product Code:
AG-40T-0022
Product Group:
Recombinant Proteins
Regulatory Status:
RUO
Target Species:
Human
Product Types:
  • Enzymes
  • Tagged
Shipping:
Dry Ice
Storage:
Short term: -20°C. Long term: -80°C
 

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This product comes from: Switzerland.
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  • Further Information
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Further Information

Alternate Names/Synonyms:
Poly(ADP-ribose) Glycohydrolase
Biological Activity:
~100ng is required for poly(ADP-ribose) degradation assays.
Concentration:
Lot dependent (0.1-1.5mg/ml)
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 0.2% NP-40, 50mM imidazole and 10% glycerol.
Handling Advice:
After opening, prepare aliquots and store at -80°C.Avoid freeze/thaw cycles.
Long Description:
Protein. Human PARG is fused to a His-tag. Source: Sf21 cells. Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 0.2% NP-40, 50mM imidazole and 10% glycerol. Purity: >95% (SDS-PAGE). Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by poly(ADP-ribose) glycohydrolase. PARG hydrolyzes poly(ADP-ribose) at glycosidic (1''-2') linkage of ribose-ribose bond to produce free ADP-ribose.
NCBI, Uniprot Number:
Q86W56
Package Type:
Plastic Vial
Product Description:
Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by poly(ADP-ribose) glycohydrolase. PARG hydrolyzes poly(ADP-ribose) at glycosidic (1''-2') linkage of ribose-ribose bond to produce free ADP-ribose.
Purity:
>95% (SDS-PAGE)
Sequence:
Human PARG is fused to a His-tag.
Source / Host:
Sf21 cells
TAGs:
His
Transportation:
Non-hazardous
UNSPSC Category:
Other Proteins
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 6 months after receipt when stored at -80°C.

References

Macrodomain-containing proteins are new mono-ADP-ribosylhydrolases: F. Rosenthal, et al.; Nat. Struct. Mol. Biol. 20, 502 (2013) | Liquid demixing of intrinsically disordered proteins is seeded by poly(ADP-ribose): M. Altmeyer, et al.; Nat. Commun. 6, 8088 (2015) | 5-diphosphoribose is an endogenous TRPM2 superagonist: R. Fliegert, et al.; Nat. Chem. Biol. 13, 1036 (2017) | PARP1 catalytic variants reveal branching and chain length-specific functions of poly(ADP-ribose) in cellular physiology and stress response: L. Aberle, et al.; Nucleic Acid Res. 48, 10015 (2020) | The Ubiquitin Ligase TRIP12 Limits PARP1 Trapping and Constrains PARP Inhibitor Efficiency: M. Gatti, et al.; Cell Rep. 32, 107985 (2020)