Chemodex

5(6)-CFDA

Product Code:
 
CDX-C0046
Product Group:
 
Dyes, Stains, and Probes
Supplier:
 
Chemodex
Regulatory Status:
 
RUO
Shipping:
 
Ambient
Storage:
 
Short term: +4°C. Long term: +4°C
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Chemical Structure
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Chemical Structure

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CDX-C0046-M02525 mg£58.00
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CDX-C0046-M100100 mg£158.00
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CDX-C0046-G0011 g£965.00
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Further Information

Alternate Names/Synonyms:
5(6)-Carboxyfluorescein diacetate
Appearance:
White powder.
CAS:
124387-19-5
EClass:
32160000
Form (Short):
liquid
Handling Advice:
Protect from light and moisture.
InChi:
InChI=1S/C24H16O7.C2H4O2/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24;1-2(3)4/h3-12H,1-2H3;1H3,(H,3,4)
InChiKey:
DCPDRLHHNBUQPJ-UHFFFAOYSA-N
Long Description:
Chemical. CAS: 124387-19-5. Formula: C25H16O9. MW: 460.39. Synthetic. CFDA is a popular, sensitive, fluorescent substrate for measuring esterase activity in live cells. It is often used in cell viability assays and "Live-Dead" assay systems, especially automated cell viability assays. The cleavage product produced upon enzymatic or chemical hydrolysis of the acetate group(s) is carboxyfluorescein which is well-retained inside live cells, while the substrate CFDA is membrane permeant.
MDL:
MFCD00467494
Molecular Formula:
C25H16O9
Molecular Weight:
460.39
Package Type:
Vial
Product Description:
CFDA is a popular, sensitive, fluorescent substrate for measuring esterase activity in live cells. It is often used in cell viability assays and "Live-Dead" assay systems, especially automated cell viability assays. The cleavage product produced upon enzymatic or chemical hydrolysis of the acetate group(s) is carboxyfluorescein which is well-retained inside live cells, while the substrate CFDA is membrane permeant.
Purity:
>90% (HPLC)
SMILES:
CC(O)=O.CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC=C3)C1=CC=C(OC(C)=O)C=C1O2
Solubility Chemicals:
Soluble in DMSO.
Source / Host:
Synthetic.
Transportation:
Non-hazardous
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at +4°C.

References

(1) C. Thrane et al.; J. Microbiol.l Meth. 28(1), 35 (1997) | (2) G. Liminga et al.; Anti-Cancer Drugs 6(4), 578 (1995) | (3) L.S. DeClerck et al.; J. Immun. Meth. 172(1), 115 (1994)