Nordic MUbio

Bromodeoxyuridine (BrdU)

Product Code:
EXA-X2834
Supplier:
Nordic MUbio
Regulatory Status:
RUO
Application:
Immunohistochemistry (IHC)
Shipping:
Ship at ambient temperature freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
 

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This product comes from: United States.
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Further Information

Applications Description:
Prior to BrdU immunostaining to detect proliferating cells it is necessary to label the cells or tissue with BrdU. Labeling can either be done in vitro for cell cultures or performed in vivo for experimental animals. Both protocols are provided.
Background:
Bromo-deoxyuridine (BrdU) is a thymidine analog that is used in cell proliferation studies. BrdU in culture is incorporated into the DNA during DNA synthesis. Cellular incorporation of BrdU can be detected by anti-BrdU specific antibodies following membrane permeabilization by flow cytometry or immunohistochemistry. The molecular weight of BrdU is 307.1.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Formulation:
Provided as 5 x 5 mg in sterile filtered solution in phosphate buffered saline
Functional Analysis:
Immunohistochemistry
Product Form:
Unconjugated
Product Stability:
See expiration date on vial

References

- M. Caroprese, M.G. Ciliberti, P. De Palo, A. Santillo, A. Sevi, M. Albenzio, 2018, Glucocorticoid effects on sheep peripheral blood mononuclear cell proliferation and cytokine production under in vitro hyperthermia,Journal of Dairy Science, ISSN 0022-0302