Nordic MUbio

Collagen Substrate FITC Conjugate

Product Code:
 
EXA-X1098
Product Group:
 
Other Proteins
Supplier:
 
Nordic MUbio
Host Type:
 
Bovine (Cattle)
Regulatory Status:
 
RUO
Shipping:
 
Ship at ambient temperature freeze upon arrival
Storage:
 
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
 

No additional charges, what you see is what you pay! *

CodeSizePrice
EXA-X109810 mg£553.00
This product currently has a longer than usual lead time. Please contact us for further information.
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges to UK mainland customers, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 7-10 working days.
Contact us for more accurate information.
  • Further Information
  • References
  • Show All

Further Information

Applications Description:
Used for the assay of mammalian collagenase activity. To heat denature collagen for preparing a substrate for gelatinase, transfer collagen solution to a brown glass bottle and heat to 80°C.
Background:
FITC-labeled bovine type I Collagen Substrate is an excellent substrate for examining collagenase activity. This labelled substrate is highly purified and telo-peptide free and is supplied lyophilized. To minimize background levels in collagenase assays, FITC-labeled collagen has been enzymatically pre-treated and further purified by ion-exchange chromatography. FITC-labeled collagen can also be used as a substrate for cell culture. Collagen degradation products in the culture supernatants can be directly determined by measured the flourescence at 520 nm (Emission)/490 nm (Excitation).
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Cellular Structures
Formulation:
Provided as a 1 mg/ml solution in 0.01M acetic acid.
Functional Analysis:
Activity Assay
Immunogen:
Bovine skin
Label:
FITC
Product Form:
FITC
Product Stability:
Stable in acidic buffer when stored at -20°C, however slowly degrades when stored in under neutral conditions.
Purity:
>95%
Synonyms:
Extracted from bovine skin

References

1. Terato, K., et al. 'A rapid assay method of collagenase activity using 14C-labeled soluble collagen as substrate.' Biochim. Biophys. Acta 1976, 445, 753-762

2. Sellers, A., et al. 'Evidence that latent collagenases are enzyme-inhibitor complexes.' Biochem. J. 1977, 163, 303-307

3. Shinkai, H., et al. 'A complex of collagenase with low molecular weight inhibitors in the culture medium of embryonic chick skin explants.' J. Biochem. (Tokyo) 1977, 81, 261-263

4. Shinkai, H. & Nagai, Y., 'A latent collagenase from embryonic human skin explants.' J. Biochem. (Tokyo) 1977, 81, 1261-1263

5. Murawaki, Y., et al. 'Serum collagenase activity in patients with chronic liver disease.' J. Hepatol. 1993, 328-334