ProSci

KLK5 Antibody

Product Code:
PSI-18-897
Product Group:
Primary Antibodies
Supplier:
ProSci
Host Type:
Rabbit
Antibody Isotype:
IgG
Antibody Clonality:
Polyclonal
Regulatory Status:
RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using KLK5 antibody (18-897) at 1:400 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
2 / 3
Immunohistochemistry of paraffin-embedded rat kidney using KLK5 antibody (18-897) at dilution of 1:200 (40x lens).
3 / 3
Immunohistochemistry of paraffin-embedded human liver cancer using KLK5 antibody (18-897) at dilution of 1:200 (40x lens).
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Western blot analysis of extracts of various cell lines, using KLK5 antibody (18-897) at 1:400 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using KLK5 antibody (18-897) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using KLK5 antibody (18-897) at dilution of 1:200 (40x lens).

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This product comes from: United States.
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Further Information

Additional Names:
KLK5, kallikrein-related peptidase 5, KLK-L2, KLKL2, SCTE, kallikrein 5, kallikrein-like protein 2, stratum corneum tryptic enzyme, UNQ570/PRO1132
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its expression is up-regulated by estrogens and progestins. The encoded protein is secreted and may be involved in desquamation in the epidermis. Alternative splicing results in multiple transcript variants encoding the same protein.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide of human KLK5
NCBI Gene ID #:
25818
NCBI Official Name:
kallikrein-related peptidase 5
NCBI Official Symbol:
KLK5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 32kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Signal Transduction
Swissprot #:
Q9Y337
User NOte:
Optimal dilutions for each application to be determined by the researcher.