CGT Global

Peripheral Blood CD19+ CD27+

Product Code:
PB192702C
Supplier:
CGT Global
Sample Source:
Peripheral Blood
Sample Type:
Isolated Cells
Species:
Human
Host Type:
Human
Regulatory Status:
RUO
Shipping:
Cryopreserve
 

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This product comes from: United States.
Typical lead time: 14-21 working days.
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Further Information

Description:
Peripheral blood CD19+CD27+ memory B cells become active in the wake of a primary infection from a particular antigen. Memory B cells can persist for years or even a lifetime after an encounter with an antigen. The presence of these cells allows for a faster antibody response if the host comes into contact with the same pathogen.
Human CD19+CD27+ memory B cells are isolated from peripheral blood mononuclear cells. Cells expressing CD2, CD14, CD16, CD36, CD43, and CD235a are depleted from the mononuclear cell population using immunomagnetic particles leaving an enriched CD19+ fraction. CD27+ cells are then enriched from the CD19+ fraction using immunomagnetic anti-CD27 particles leaving purified CD19+CD27+ memory B cells. Isolated cells are characterized by flow cytometry before cryopreservation to ensure a highly pure and viable cell population.
Cells are obtained using Institutional Review Board (IRB) approved consent forms and protocols in CLIA Certified clinics registered by the FDA.
Positive, Negative or Cultured?:
Negative or Positive