SARS-CoV-2 RT-qPCR Detection Kit (RUO)

Coronaviruses are a family of large RNA viruses with size ranging from 26 to 32 kb. These viruses are zoonotic and in human can cause respiratory infections. As the coronavirus is an RNA virus it has a relatively high mutation rate resulting in rapid evolution. In December 2019, a new deadly coronavirus known as SARS-CoV-2 (previously known as 2019-nCoV), which has a high sequence similarity to SARS-CoV, was identified and has caused a pneumonia, known as Covid-19, outbreak in Wuhan, China and spread globally. ScienCell's SARS-CoV-2 RT-qPCR Detection Kit (SCVPD) is designed to detect the presence of SARS-CoV-2 Coronavirus in respiratory specimens and serum samples following CDC guidelines (see Appendix 1). One-step TaqProbe RT-qPCR master mix is conveniently included in the kit. Please read carefully and follow these guidelines for biosafety precautions when dealing with possible infectious samples and for obtaining quality data. Included in the kit are two primer/probe sets (Cat #7038-N1 and 7038-N2) that target coronavirus SARS-CoV-2 nucleocapsid (N) gene, and the Human RPP30 gene primer/probe set (Cat #7038-RP) that targets exon 1 of human RPP30 gene and serves as a control to assess specimen quality. In addition, a non-infectious positive control (Cat #7038-Pos) and nuclease-free water are included in the kit. The positive control (Cat #7038-Pos) consists of non-infectious viral RNA fragments spiked into human small airway epithelial cells and serves to ensure reagents and instruments are working properly. Please refer to Tables 4 and 5 for results interpretation.

ScienCell's SARS-CoV-2 RT-qPCR Detection Kit (SCVPD) is designed to detect the presence of SARS-CoV-2 Coronavirus in respiratory specimens and serum samples following CDC guidelines. One-step TaqProbe RT-qPCR master mix is conveniently included in the kit. For Research Use Only. Not for use in diagnostic procedures.

Kit Components:

• #MB802a - One-step TaqProbe RT-qPCR master mix, 4x - 1.5 mL
• #7038-N1 - N gene primer/probe set 1, in solution - 200 ul
• #7038-N2 - N gene primer/probe set 2, in solution - 200 ul
• #7038-RP - Human RPP30 gene primer/probe set, in solution - 200 ul
• #7038-H2O - Nuclease-free H2O - 8 mL
• #7038-Pos - Positive control (non-infectious;RNA: 500 - 1000 copies/ul, cells: 200 - 300 counts/ul)) - 200 ul