0.2% Heparin

Heparin is a 10-12 kDa polysaccharide produced in mast cells. Heparin inhibits blood coagulation through binding and activation of anti-thrombin [1] and acts as a cofactor to basic Fibroblast Growth Factor-2 (bFGF) [2, 3, 4]. The effect of heparin on cell growth and morphology in vitrois cell-type dependent and may promote or inhibit cell proliferation. Culture media may be supplemented with heparin solution (HEP) at 1:1000-1:100 (0.36 - 3.6 U/mL). The optimal concentration should be determined for individual cell types and culture conditions. Concentration - 2 mg/mL heparin sodium salt (from porcine intestinal mucosa) in Dulbecco's Phosphate-Buffered Saline, sterile-filtered. Potency: > 180 Units per milligram*. *Units are USP (United States Pharmacopeia units) and indicate heparin potency by weight. Units are not evaluated by ScienCell but are provided by supplier.

HEP is for research use only. It is not approved for human or animal use, or for application in clinical or in vitrodiagnostic procedures.