ScienCell's GeneQuery™ Human Cell Junctions qPCR Array Kit (GQH-JXN) profiles 88 key genes involved in cell junction formation, organization, and maintenance. This kit surveys cell-cell junctions that are important for cellular communication, paracellular transport, and anchoring. Brief examples of how included genes may be categorized are shown below:
- Gap junctions: - AP2M1, CLTA, CLTCL1, GJA1, GJB1, PANX1
- Adherens junctions: - ANG, CADM1, CDH2, CDH5, FAT2, NECTIN2
- Tight junctions: - CDX2, CLDN1, CLDN5, JAM2, SNAI1, TJP1 (ZO-1)
- Desmosomes: - DSC1, DSG1, DSP, ITGB4, JUP
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg^2+ and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
This kit profiles 88 key genes involved in cell junction formation, organization, and maintenance. This kit surveys cell-cell junctions that are important for cellular communication, paracellular transport, and anchoring.
