Sciencell

SARS-CoV-2 SYBR® Green RT-qPCR Quantification Kit

Product Code:
 
SC-RU7058
Product Group:
 
PCR Kits
Supplier:
 
Sciencell
Regulatory Status:
 
RUO
Shipping:
 
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SC-RU7058100 reactions£448.00
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Further Information

Description:
ScienCell's SARS-CoV-2 SYBR® Green qPCR Quantification Kit (SVSGQ) is designed to purify and quantify the SARS-CoV-2 coronavirus presence in samples. Four primer sets are included in the kit. Two of them (Cat #7058a and 7058b) target coronavirus SARS-CoV-2 nucleocapsid (N) gene and spike (S) gene, respectively. The human ACE2 gene primer set (Cat #7058c) targets human angiotensin-converting enzyme 2 (ACE2) gene, which serves as a cellular receptor for SARS-CoV-2. The human ACTB gene primer set (Cat #7058d) targets human β-actin (ACTB) housekeeping gene, which serves as a normalization control for cell number quantification. All primer sets are verified to possess high specificity and efficiency near 100%, with no primer dimer formation under recommended PCR conditions. Additionally, a non-infectious positive control (Cat #7058-Pos) and nuclease-free water (Cat #GQ100-4) are included in the kit. The positive control (Cat #7058-Pos) consists of non-infectious RNA fragments spiked with human small airway epithelial cells and serves to ensure reagents and instruments are working properly.
Extra Description:
ScienCell's SARS-CoV-2 SYBR® Green qPCR Quantification Kit (SVSGQ) is designed to purify and quantify the SARS-CoV-2 coronavirus presence in samples. For Research Use Only. Not for use in diagnostic procedures.

Kit Components:

  • #MB6018a-1 - GoldNStart TaqGreen qPCR Master Mix - 1 mL x 4
  • #7058a -SARS-CoV-2 N gene primer set, in solution - 200 uL
  • #7058b - SARS-CoV-2 S gene primer set, in solution - 200 uL
  • #7058c - Human ACE2 gene primer set, in solution - 200 uL
  • #7058d - Human ACTB gene primer set, in solution - 200 uL
  • #GQ100-4 - Nuclease-free H2O - 4 mL
  • #7058-Pos - Positive control (non-infectious;RNA: 500 - 1000 copies/uL, cells: 200 - 300 counts/uL) - 200 uL

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