Double stranded RNA control Poly(I:C)
Product Code:
SCI-10090100
SCI-10090100
Product Group:
Assay Supporting Tools and Kit Components
Assay Supporting Tools and Kit Components
Supplier:
Nordic MUbio
Nordic MUbio
Regulatory Status:
RUO
RUO
Applications:
- Dot Blot
- Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
Ship on blue ice
Ship on blue ice
Storage:
Store at -20 °C to -80 °C upon arrival, avoid freeze/thaw cycles, store in aliquots.
Store at -20 °C to -80 °C upon arrival, avoid freeze/thaw cycles, store in aliquots.
No additional charges, what you see is what you pay! *
Stay in control of your spending. These prices have no additional charges to UK mainland customers, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 7-10 working days.
Contact us for more accurate information.
Typical lead time: 7-10 working days.
Contact us for more accurate information.
- Further Information
- Show All
Further Information
Applications Description:
Double stranded RNA control Poly(I:C) is suitable for use as a size standard in dsRNA and RNAi analysis on both non-denaturing polyacrylamide and agarose gels. We suggest to use it as a homogenous, well-characterized positive control for different dsRNA detection methods, e.g. for ELISA and dot-blots.
The optimum working dilution of the Poly(I:C) dsRNA control for any specific application should be established by titration.
Background:
The low molecular weight Poly(I:C) dsRNA sequence is intended to be used as a positive control in double-stranded (ds)RNA detection. The sequence does not contain any viral or potentially transforming elements.
Positive control for dsRNA detection by anti dsRNA monoclonal J2, J5, K1 and K2 antibodies.
Caution: The Poly(I:C) dsRNA positive control, to be combined with our monoclonal anti dsRNA antibodies J2, J5, K1 and K2 in both the direct AN-ELISA and sandwich ELISA, is not intended to be used as a quantitative standard for other dsRNA preparations. The anti dsRNA antibodies may exhibit a different degree of reactivity with different dsRNAs obtained from natural sources. It is therefore only intended to be used as a positive control to see if the ELISA has been executed correctly and that the test shows a linear relationship between the amount of dsRNA and the read out, for example the OD450.
Field of Interest:
Infectious Agents & Disease|Microbiology
Functional Analysis:
Direct ELISA and Sandwich ELISA (relative activity to reference antigen)
Product:
Each vial contains 50 ul of a 1 mg/ml Poy(I:C) solution in RNAse/DNAse-free STE Buffer (0.1 M NaCl, 1 mM EDTA, 50 mM Tris-HCl, pH 7.0).
Low molecular weight polyinosine-polycytidylic acid (i.e. poly(I:C)) is a synthetic analog of double-stranded RNA (dsRNA) and is comprised of short strands of inosine poly(I) homopolymer annealed to strands of cytidine poly(C) homopolymer. The average size of Poly(I:C) LMW is 0.2 kb to 1 kb.
Product Stability:
Shelf Life: 24 months
Purity:
Absorbance spectrum
Source:
In vitro synthesized RNA (heteropolymer consisting of unmodified ribonucleotides)
Synonyms:
Double stranded RNA (dsRNA) control/Poly(I):Poly(c)