ProSci

SARS-CoV-2 WT Pseudovirus

Product Code:
PSI-95-200
Product Group:
Transduction Tools
Supplier:
ProSci
Regulatory Status:
RUO
Applications:
  • Neutralisation
  • Viral Infection
Shipping:
Dry Ice
Storage:
The pseudovirus is stable in a liquid state at -70˚ for 12 months. Avoid repeated freeze-thaw cycles.
 

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This product comes from: United States.
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Further Information

Additional Names:
Spike glycoprotein Receptor-binding domain, S glycoprotein, Spike protein, pseudovirus, pseudovirus reporter, reporter virus particle
Amount:
1.0 mL (0.5mL X 2 vials) for two 96-well plates
Application Note:

Protocol: click here to download

1. Require BSL-2 or higher working environment
2. 5 ?L per application (96-well plate) with at least 0.3 X 106 RLU / well.
3. Avoid freezing and thawing cycle to ensure the titer of the pseudovirus.
4. This product is for Research Use only
5. Neutralization assay protocol is available
Background:
SARS-CoV-2 Pseudovirus uses HIV pseudotyped with SARS-CoV-2 spike protein and firefly luciferase reporter. This pseudovirus effectively infects human ACE2-overexpressing cells and can be used to screen for inhibitors of Spike-ACE2 interaction, determine neutralizing antibody titers, and is a valuable tool for COVID-19 vaccine development as well.
Buffer:
DMEM/10% FBS
NCBI Gene ID #:
43740568
NCBI Official Name:
Surface glycoprotein
NCBI Official Symbol:
S
NCBI Organism:
Severe acute respiratory syndrome coronavirus 2
Physical State:
Liquid
Protein Accession #:
QHD43416
Protein GI Number:
1791269090
Recombinant Protein Sequence:
SARS-CoV-2 Spike Met 1 - Cys 1255
Research Area:
Infectious Disease, COVID-19
Source:
HEK293 cells
Swissprot #:
P0DTC1

Documents