Yersinia pestis (Y. pestis)is a non-spore-forming, Gram-negative, non-motile, and rod-shaped bacterium. It can infect humans and other mammals via rat fleas and causes pneumonic, septicemic, and bubonic plague. Plague can be transmitted among humans and mammals by contacting with contaminated fluid or tissue or breathing in Y. pestis -containing droplets. ScienCell's Y. pestisBacterium qPCR Detection Kit (PLAG) is designed to detect the presence of Y. pestisbacterium in extracted DNA samples. The plague primer set (Cat #7088-PLAG) included in the kit targets Y. pestisgenome. It is verified to have high specificity and efficiency near 100% under recommended PCR conditions. In addition, a non-infectious positive control (Cat #7088-Pos) and nuclease-free water (Cat #GQ100-4) are included in the kit. The positive control consists of non-infectious DNA fragments and serves to ensure reagents and instruments are working properly.
Yersinia pestis (Y. pestis)is a non-spore-forming, Gram-negative, non-motile, and rod-shaped bacterium. ScienCell's Y. pestisBacterium qPCR Detection Kit (PLAG) is designed to detect the presence of Y. pestisbacterium in extracted DNA samples. For Research Use Only. Not for use in diagnostic procedures.
Kit Components:
- #MB6018a-1 - 2X GoldNStart TaqGreen qPCR master mix - 1 mL - Storage: -20°C
- #7088-PLAG - Plague primer set, in solution - 200 µL - Storage: -20°C
- #7088-Pos - Positive control (non-infectious DNA: 1,000-2,000 copies/µL) - 200 µL - Storage: -80°C
- #GQ100-4 - Nuclease-free H2O - 4 mL - Storage: 4°C
