Abnova Corporation

RNase Inhibitor

Product Code:
U0439
Regulatory Status:
RUO
Target Species:
Mouse
Applications:
  • Functional Study
  • In vitro transcription (IVT)
Storage:
Store at -20°C.
 

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This product comes from: Taiwan.
Typical lead time: 14-21 working days.
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Further Information

Note:
It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus.
Compared to human RNase inhibitors, murine RNase inhibitor does not contain two cysteines that are highly sensitive to oxidation which causes inactivation of the inhibitor. That making it stable at low concentrations of DTT (less than 1 mM).
Preparation Method:
Escherichia coli expression system
Product Description:
Murine RNase inhibitor binds to RNase A, B or C in a 1:1 ratio through non-covalent bonding, thereby inhibiting the activity of the enzymes and protecting RNA from degradation.
Quality Control Testing:
Exonuclease Activity: 40 U of murine RNaseinhibitor with 1 ug Lambda-Hind III digest DNA at 37°C for 16 hours yields no degradation as determined by agarose gel electrophoresis.
Endonuclease Activity: 40 U of murine RNase inhibitor with 1 ug Lambda DNA at 37°C for 16 hours yields no degradation as determined by agarose gel electrophoresis.
Nicking Activity: 40 U of murine RNase inhibitor with 1 ug pBR322 at 37°C for 16 hours yields no degradation as determined by agarose gel el
Storage Buffer:
In 50 mM KCl, 20 mM HEPES-KOH, pH 7.6 (8 mM DTT and 50% glycerol)