RevMAb Biosciences

5-Methylcytosine (5-mC) DNA Matched Antibody Pair

Product Code:
 
REV-31-1110-MK
Product Group:
 
Antibody Pairs
Supplier:
 
RevMAb Biosciences
Regulatory Status:
 
RUO
Target Species:
 
Species Independent
Shipping:
 
Blue Ice
Storage:
 
Short term: +4°C. Long term: -20°C
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Detection of 5-Methylcytosine in mouse different tissues genomic DNA. Sandwich ELISA using anti-5-Methylcytosine (clone RM231) as the capture antibody (50ng/well at 1ug/mL), and biotinylated anti-Adenine and Guanine (clone RM476) as the detection antibody (0.2ug/mL), followed by an AP-conjugated streptavidin.
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Detection of 5-Methylcytosine in mouse different tissues genomic DNA. Sandwich ELISA using anti-5-Methylcytosine (clone RM231) as the capture antibody (50ng/well at 1ug/mL), and biotinylated anti-Adenine and Guanine (clone RM476) as the detection antibody (0.2ug/mL), followed by an AP-conjugated streptavidin.

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REV-31-1110-MK-KI011 Set£764.00
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This product comes from: Switzerland.
Typical lead time: 14-21 working days.
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Further Information

Alternate Names/Synonyms:
5-mC
Assay Type:
Sandwich
Detection Type:
Colorimetric
EClass:
32160000
Form (Short):
liquid
Handling Advice:
Avoid freeze/thaw cycles.
Kit Contains:
1) Capture Antibody: Anti-5-Methylcytosine Rabbit Monoclonal Antibody, Clone RM231, 50 ug at 1.0 mg/mL in 50% Glycerol/PBS with 0.09% sodium azide, without BSA or other carrier proteins.2) Detection Antibody: Biotin Anti-Adenine and Guanine Rabbit Monoclonal Antibody, Clone RM476, 25 ug at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide;
Long Description:
Matched Pair Detection Set. This antibody pair detects 5-methylcytosine in denatured DNA (single strand DNA). It does not react to non-methylated cytosine and other modified cytosine (5-hmC, 5-caC, or 5-fC) in DNA. This product includes capture and detection antibodies for Sandwich ELISA assays.
Package Type:
Vial
Product Description:
5-Methylcytosine is a methylated form of the DNA base cytosine that regulates gene transcription and is involved in epigenetics. It is one of the best known epigenetic modifications. This methylation typically occurs at cytosine in CpG dinucleotides in vertebrates. When cytosine is methylated, the DNA maintains the same sequence, but the expression of methylated genes can be altered. 5-Methylcytosine is a very important repressor of transcription in the genome. When present in promoters, 5-Methylcytosine is associated with stable, long-term transcriptional silencing. This may occur by either blocking positive transcription factors, or promoting the binding of negative ones.
Specificity:
This antibody pair detects 5-methylcytosine in denatured DNA (single strand DNA). It does not react to non-methylated cytosine and other modified cytosine (5-hmC, 5-caC, or 5-fC) in DNA. This product includes capture and detection antibodies for Sandwich ELISA assays.
Transportation:
Non-hazardous
UNSPSC Number:
41116126
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C.

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