Protein G Sepharose Purification Kit

Affinity purified Protein G antibodies, developed in mice, were conjugated to NHS-sepharose. Protein G is a 65 kDa (G148 Protein G) and a 58 kDa (C40 Protein G) surface protein found in the cell wall of Staphylococcus aureus. Like Protein A, Protein G also has a high affinity to Human IgG, Mouse IgG, and others and is, therefore, suitable for purification and detection of immunoglobulins. Therefore, Croyez Protein G sepharose is an efficient technique for IgG purification. Recombinant protein G is immobilized at over 5 mg per mL of 50% slurry, and this kit allows a rapid and efficient affinity purification of active IgG proteins. The affinity resin allows an efficient binding of IgG proteins without the need for preliminary steps and calibrations. The affinity-bound IgG proteins can be efficiently eluted from the resin by acid condition. The eluted IgG proteins can be used for characteristic analysis.