Anti-V5 Tag Antibody Sepharose Purification Kit

Affinity purified anti-V5 antibodies, developed in mice, were conjugated to NHS-sepharose. It is an efficient technique for isolating recombinant proteins or mammalian expression proteins. The V5 epitope system relies on the peptide (N-Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-C), which is able to react with N- and C- terminal V5-tagged fusion proteins and may be used for immunoprecipitation or immune affinity purification. The purified antibody is immobilized at 3-5 mg antibody per mL of 50% slurry, and this kit allows a rapid and efficient affinity purification of active V5 fusion proteins. The affinity resin allows an efficient binding of V5 fusion proteins without the need for preliminary steps and calibrations. The affinity bound V5 fusion proteins can be efficiently eluted from the resin by acid condition. The eluted proteins can be used for characteristic analysis.