Anti-EGFP Antibody Sepharose Purification Kit

Anti-EGFP sepharose consists of an EGFP VHH coupled to NHS-sepharose. It is an efficient technique for isolating recombinant proteins or mammalian expression proteins. The EGFP epitope system relies on a VHH EGFP recombinant antibody, which is able to react with N- and C- terminal GFP tagged and EGFP tagged fusion proteins. This product can be used for immunoprecipitation or immune affinity purification. The purified antibody is immobilized at 3-5 mg antibody per mL of 50% slurry, and this kit allows a rapid and efficient affinity purification of active EGFP fusion proteins. The affinity resin allows an efficient binding of EGFP fusion proteins without the need for preliminary steps and calibrations. The affinity bound EGFP fusion proteins can be efficiently eluted from the resin by acid condition. The eluted proteins can be used for characteristic analysis.