Leinco Technologies

HEPES buffered saline with EDTA (10X)

Product Code:
LEI-H1679
Product Group:
Biological Buffers
Regulatory Status:
RUO
Storage:
Store Leinco sheath fluids at room temperature. DO NOT FREEZE. Stable when stored as directed for 12 months from date received.
 

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This product comes from: United States.
Typical lead time: 14-21 working days.
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Further Information

Long Description:
HEPES is a zwitterionic (?hybrid?) organic chemical buffering agent; one of the twenty Good?s Buffers excellence in early discovery research HEPES Buffered Saline with EDTA (10X) Cell Culture Buffer & Flow Cytometry Sorting Reagents which derives from a set of buffers described by Dr. Norman Good and his colleagues in 1966 (Good et.al., Biochemistry 1966). HEPES is widely used in cell culture because it is better at maintaining a physiological pH compared to bicarbonate buffers and provides extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO2 incubator. "Good?s Buffers" and HEPES are associated with the following characteristics: - pKa values between 6.0 and 8.0 - high solubility - membrane impermeability - limited effect on biochemical reactions - very low visible and ultraviolet light absorbance (great for Flow Cytometry applications) - chemically and enzymatically stable