Leinco Technologies

Anti-Human CD16 (Clone 3G8) - Purified in vivo GOLD™ Functional Grade

Product Code:
LEI-C2858
Product Group:
Primary Antibodies
Host Type:
Mouse
Antibody Isotype:
IgG1 κ
Antibody Clonality:
Monoclonal
Antibody Clone:
3G8
Regulatory Status:
RUO
Target Species:
  • Baboon
  • Chimpanzee
  • Cynomolgus Monkey
  • Human
  • Marmoset
  • Pigtailed macaque
  • Rhesus Macaque
  • Squirrel monkey
Applications:
  • Blocking
  • Flow Cytometry
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunoprecipitation (IP)
  • In Vivo Assay
  • Mass Cytometry (CyTOF)
Product Type:
in vivo
Shipping:
2-8°C
Storage:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
 

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Further Information

Antigen Distribution:
CD16 is expressed on NK cells, activated monocytes, macrophages, neutrophils, and placental trophoblasts.
Concentration:
? 5.0 mg/ml
Conjugate/Tag/Label:
in vivo GOLD™, Purified in vivo Functional Grade
Format:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Immunogen:
Human PMN cells
Long Description:
CD16 antibody, 3G8, recognizes human CD16, also known as low-affinity IgG receptor III (FcγRIII). There are two distinct forms of CD16, CD16a (FcγRIIIa) and CD16b (FcγRIIIb). CD16a is a 50-65 kDa heterooligomeric polypeptide-anchored transmembrane protein expressed by NK cells, macrophages, and subsets of monocytes1. CD16b is a 48 kDa monomeric glycosylphosphatidylinositol (GPI)-anchored protein expressed on neutrophils1. Upon binding to the Fc portion of IgG or IgG-antigen complex, both CD16 isoforms induce signaling cascades resulting in multiple functions, including phagocytosis, cytokine release, proliferation, degranulation, and antibody dependent cell-mediated cytotoxicity (ADCC)2.
NCBI Gene:
2214
Purity:
?95% monomer by analytical SEC, >95% by SDS Page
Target:
CD16

References

1. Ravetch JV & Perussia B. (1989) J Exp Med. 170(2):481-497 2. Nimmerjahn F & Ravetch JV (2008) Nat Rev Immunol. 8(1):34-47