Elabscience

Bradford Protein Colorimetric Assay Kit

Product Code:
E-BC-K168-S
Product Group:
Biochemical Assay Kits
Supplier:
Elabscience
Regulatory Status:
RUO
Application:
Quantification
Shipping:
Ice packs
Storage:
Reagent1:2-8°C;Reagent2:-20°C
1 / 2
2 / 2
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This product comes from: China.
Typical lead time: 14-21 working days.
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  • Further Information
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Further Information

Assay Time:
50 min
Detection Instrument:
Spectrophotometer
Detection Range:
0.026-1.2 mg/mL
Detection Type:
Colorimetric method
Inter cv:
2.3
Intra cv:
1.8
Result Type:
Quantitative
Sensitivity:
0.026 mg/mL
Target Synonym:
TP
Test Principle:
Coomassie brilliant blue G-250 is red under the free state, and it has the maximum absorbance at 465 nm. When the Coomassie brilliant blue G-250 combined to protein, the compound will have the maximum at 595 nm. The absorbance value is directly proportional to the protein content, so the concentration of total protein can be calculated directly by measuring the OD value at 595 nm.
Tested Sample Types:
Serum,plasma,animal tissue

Documents

References

  • https://www.nature.com/articles/s41598-024-64577-5
  • https://journals.sagepub.com/doi/abs/10.1177/09731296241297429
  • https://pubs.rsc.org/en/content/articlelanding/2024/fo/d4fo01061b
  • https://www.jstage.jst.go.jp/article/jos/71/9/71_ess22033/_article/-char/ja/