Elabscience

Total Cholesterol (TC) Colorimetric Assay Kit (Single Reagent, COD-PAP Method)

Product Code:
 
E-BC-K109-M
Product Group:
 
Biochemical Assay Kits
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Application:
 
Quantification
Shipping:
 
Ice packs
Storage:
 
2-8°C
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E-BC-K109-M-48T48T£143.00
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E-BC-K109-M-96T96T£184.00
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E-BC-K109-M-500Assays500Assays£403.00
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  • Further Information
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Further Information

Assay Time:
20 min
Detection Instrument:
Microplate reader
Detection Range:
0.29-25.85 mmol/L
Detection Type:
Colorimetric method
Inter cv:
8.3
Intra cv:
3.1
Result Type:
Quantitative
Sensitivity:
0.29 mmol/L
Target Synonym:
TC
Test Principle:
Total cholesterol includes free cholesterol and cholesterol esters. Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce δ4-cholestenone and hydrogen peroxide. In the presence of 4-aminoamylpyridine and phenol, hydrogen peroxide catalyze peroxidase to form red quinone compounds of benzoquinone imine phenizone.The color depth of the generated quinone is directly proportional to the cholesterol content. The absorbance values of the standard tube and the sample tube are measured respectively, and the cholesterol content in the sample can be calculated.
Tested Sample Types:
Serum,plasma,tissue

Documents

References

  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7429390/
  • https://www.mdpi.com/2072-6643/15/17/3779
  • https://www.cell.com/heliyon/fulltext/S2405-8440(24)12196-2
  • https://journals.sagepub.com/doi/abs/10.1177/09731296241281421
  • https://journals.sagepub.com/doi/abs/10.1177/09731296241297429
  • https://www.tandfonline.com/doi/full/10.1080/10520295.2024.2344491