Elabscience

CuZn/Mn Superoxide Dismutase (CuZn-SOD/Mn-SOD) Activity Assay Kit (Hydroxylamine Method)

Product Code:
 
E-BC-K022-S
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Application:
 
Activity Assay
Shipping:
 
Ice packs
Storage:
 
Reagent4:-20°C;Others:2-8°C
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E-BC-K022-S-50Assays50Assays£198.00
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E-BC-K022-S-100Assays100Assays£252.00
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  • Further Information
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Further Information

Assay Time:
100 min
Detection Instrument:
Spectrophotometer
Detection Range:
2.03-155 U/mL
Detection Type:
Colorimetric method
Inter cv:
7.2
Intra cv:
4
Result Type:
Enzyme Activity
Sensitivity:
2.03 U/mL
Target Synonym:
CuZn-SOD;T-SOD
Test Principle:
Superoxide anion (O2-) produced by xanthine and xanthine oxidase system can oxidize hydroxylamine to form nitrite which appear purplish red after chromogenic reaction. The SOD in the sample has a specific inhibitory effect on superoxide anion (O2-), can reduce the content of nitrite. The OD value is lower than control and the activity of SOD is calculated through the formula. There are two kinds of SOD (CuZn-SOD, Mn-SOD) in the cells of higher animals, and the sum of them is equal to the total SOD. The activity of Mn-SOD in the sample will lost after sample pretreatment, but the activity of CuZn-SOD will not.
Tested Sample Types:
Serum,plasma,urine,cells,cell culture supernatant,tissue

Documents

References

  • https://www.mdpi.com/1963432
  • https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8908175/
  • https://onlinelibrary.wiley.com/doi/abs/10.1111/jphp.13319
  • https://www.mdpi.com/2103898
  • https://onlinelibrary.wiley.com/doi/abs/10.1002/tox.24274
  • https://europepmc.org/article/med/38628099?javascript_support=no