Elabscience

Choline Acetyltransferase (ChAT) Activity Assay Kit (Tissue Samples)

Product Code:
 
E-BC-K125-S
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Application:
 
Activity Assay
Shipping:
 
Ice packs
Storage:
 
Reagent236 -20°C;Others:2-8°C
 

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CodeSizePrice
E-BC-K125-S-50Assays50Assays£198.00
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E-BC-K125-S-100Assays100Assays£252.00
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  • Further Information
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Further Information

Assay Time:
80 min
Detection Instrument:
Spectrophotometer
Detection Range:
1.21-40 U/g fresh wet weight
Detection Type:
Colorimetric method
Inter cv:
9.2
Intra cv:
5
Result Type:
Enzyme Activity
Sensitivity:
1.21 U/g fresh weigh
Target Synonym:
ChAT
Test Principle:
Acetyl-CoA can react with choline under the catalysis of choline acetyltransferase (ChAT) to produce coenzyme A (CoA), CoA can combine with the 4, 4-dithiopyridine. The activity of ChAT can be calculated indirectly by measuring the OD value at 324 nm.
Tested Sample Types:
Animal tissue

Documents

References

  • https://www.mdpi.com/2076-3921/10/6/835
  • https://www.mdpi.com/2076-3921/9/7/620
  • https://link.springer.com/article/10.1007/s11064-022-03684-z
  • https://www.mdpi.com/2050368
  • https://link.springer.com/article/10.1007/s13596-022-00653-3