Elabscience

Adenosine Deaminase (ADA) Activity Assay Kit

Product Code:
 
E-BC-K197-M
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Application:
 
Activity Assay
Shipping:
 
Ice packs
Storage:
 
2-8°C
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CodeSizePrice
E-BC-K197-M-48T48T£198.00
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E-BC-K197-M-96T96T£252.00
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  • Further Information
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Further Information

Assay Time:
40 min
Detection Instrument:
Microplate reader
Detection Range:
0.03-99 U/L
Detection Type:
Colorimetric method
Inter cv:
6
Intra cv:
3
Result Type:
Enzyme Activity
Sensitivity:
0.03 U/L
Target Synonym:
ADA
Test Principle:
Adenosine deaminase (ADA) can hydrolyzed the substrate adenosine to form hypoxanthine riboside, which is hydrolyzed by purine riboside phosphatase to produce hypoxanthine and phosphate ribose. Under the action of xanthine oxidase, hypoxanthine produces hydrogen peroxide, which produces red substance under the action of peroxidase, 4-aminotepyrine and color source. The red substance has the maximum absorption peak at 550 nm and the changes of absorbance is proportional to the activity of ADA.
Tested Sample Types:
serum, plasma, animal tissue

Documents

References

  • https://pubs.rsc.org/en/content/articlelanding/2023/bm/d2bm01836e
  • https://www.sciencedirect.com/science/article/pii/S2405844021000256