Elabscience

Inhibition And Production Of Superoxide Anionic Colorimetric Assay Kit (WST-1 Method)

Product Code:
 
E-BC-K001-M
Product Group:
 
Biochemical Assay Kits
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Application:
 
Activity Assay
Shipping:
 
Ice packs
Storage:
 
Reagent3:-20°C;Others:2-8°C
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E-BC-K001-M-48T48T£198.00
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E-BC-K001-M-96T96T£252.00
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Further Information

Assay Time:
35 min
Detection Instrument:
Microplate reader
Detection Type:
Colorimetric method
Inter cv:
5.8
Intra cv:
2
Result Type:
Activity
Test Principle:
Superoxide anion free radicals are produced through the reaction system of xanthine and xanthine oxidase. WST-1 (a water-soluble tetrazolium salt) can react with the generated superoxide anion to produce water-soluble formazan. When the tested sample contains the superoxide anion free radical inhibitor, it can inhibit the formation of formazan. When the tested sample contains the substance that produces superoxide anion free radical, it can promote the formation of formazan dye. By colorimetric analysis of WST-1 products, the units of activity of inhibition or production of superoxide anion radical in samples can be calculated.
Tested Sample Types:
Serum,plasma,urine,cells,cell culture supernatant,leucocyte

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