Elabscience

Mouse IL-13(Interleukin 13) CLIA Kit

Product Code:
E-CL-M0460
Product Group:
CLIA Kits
Supplier:
Elabscience
Regulatory Status:
RUO
Target Species:
Mouse
Application:
Chemiluminescent Immunoassay (CLIA)
Shipping:
Ice packs
Storage:
An unopened kit can be stored at 2-8°C for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.
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Standard curve
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Standard curve

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Further Information

Abbreviation:
IL-13
Assay Time:
3.5h
Cross reactivity:
This kit recognizes Mouse IL-13 in samples.No significant cross-reactivity or interference between Mouse IL-13 and analogues was observed
Detection Range:
12.5-800 pg/mL
Detection Type:
Chemiluminescence
Precision:
Both intra-CV and inter-CV are < 10%.
Recovery rate:
80%-120%
Sensitivity:
7.5 pg/mL
Target Species:
Mouse
Target Synonym:
IL13;ALRH;BHR1;P600;
Test Principle:
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-13. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-13, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse IL-13. You can calculate the concentration of Mouse IL-13 in the samples by comparing the RLU value of the samples to the standard curve.
Tested Sample Types:
Serum, plasma and other biological fluids
Type:
Sandwich
UNIProt ID:
P20109
Usage:
This ELISA kit applies to the in vitro quantitative determination of Mouse IL-13 concentrations in Serum, plasma and other biological fluids.

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